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Moment of introspection aside, I’m not sure what the future holds for agents and generative AI. My use of agents has proven to have significant utility (for myself at the least) and I have more-than-enough high-impact projects in the pipeline to occupy me for a few months. Although certainly I will use LLMs more for coding apps which benefit from this optimization, that doesn’t imply I will use LLMs more elsewhere: I still don’t use LLMs for writing — in fact I have intentionally made my writing voice more sardonic to specifically fend off AI accusations.
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This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.